Getting started
TeselaGen Primer Architect designs primers for targeted-sequencing applications — amplicon panels, tiling schemes, and single PCR primer pairs. The result is a multiplex-ready primer set you can hand straight to a synthesis vendor or load into a Opentrons / Hamilton / Echo deck.
Three flows, one tool
- Gene panel — give it a list of genes (or pick a disease) and it covers every exon with multiplex-ready primers.
- Tiling scheme — for whole viral/bacterial references: overlapping amplicons across the entire sequence.
- Single PCR pair— for one region. Uses primer3 with TeselaGen's quality presets.
Your first design
- From the home page or the tool library, click Start a design.
- Pick a reference genome (GRCh38.p14 is live). The other organisms ship in M3.
- Pick a target gene — directly by symbol/alias, or via a ClinVar-curated disease association.
- On the gene track, drag to choose the base range to amplify, or leave the default of the full gene.
- Tune the scheme parameters (amplicon size, pool count, overlap) and click Design primer panel.
- After ~30 s – 5 min you land on your results page with the full primer list, pool breakdown, and amplicon map. Export to BED, TSV, CSV, or a robot-ready format.
Sign in to save your work
Guests can run the wizard end-to-end. To save designs, share them with collaborators, or reopen them later, create a free account on the sign-in page. Existing guest runs in your browser stay local — sign in before submitting to save server-side from the start.