Sequence analysis

Hairpin analyzer

Paste a DNA sequence and we'll scan it for secondary-structure hairpins, reporting their ΔG and predicted melting temperature. Useful for sanity-checking a hand-crafted primer before ordering.

Input sequence
ACGT/N characters only. Whitespace is stripped automatically. Minimum 10 nt.
0 nt cleaned· invalid (use ACGTN, ≥10 nt)